By American Academy of Ophthalmology, Robert H. Rosa Jr. MD
Part four provides fabrics in elements: half I, Ophthalmic Pathology; and half II, Intraocular Tumors: scientific features. half I makes use of a hierarchy that strikes from normal to express to aid derive a differential analysis for a particular tissue. half II is a compilation of chosen medical features of significance to the overall ophthalmologist. Following half II are the yankee Joint Committee on melanoma 2010 staging types for ocular and adnexal tumors.
Upon of entirety of part four, readers could be capable to:
Describe a based method of knowing significant ocular stipulations in line with a hierarchical framework of topography, illness strategy, basic prognosis and differential diagnosis
Summarize the stairs in dealing with ocular specimens for pathologic learn, together with acquiring, dissecting, processing, and marking tissues
Identify these ophthalmic lesions that point out systemic illness and are possibly existence threatening
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Extra resources for 2014-2015 Basic and Clinical Science Course (BCSC): Section 4: Ophthalmic Pathology and Intraocular Tumors
Limbus The limbus is a complex region of corneal, scleral, and episcleral tissues. Wounds of the limb us cause swelling in the cornea and shrinking of the sclera. Healing involves episcleral ingrowth and clear corneal fibroblastic migration. Collector channels in the sclera do not contribute to the healing. Alterations in surgical technique between clear corneal and limbal incisions may produce different healing responses. Differences include • the potential for vascular ingrowth from episcleral vessels into a limbal wound and the absence of vascularity of a clear corneal wound • surface remodeling of epithelium over a clear corneal wound that does not occur over a limbal wound Uvea Under ordinary circumstances, wounds of the iris do not stimulate a healing response in either the stroma or the epithelium.
Logistics of the biopsy i. Possible adequacy check during the biopsy (intraocular tumors) ii . Fixative to be used iii. Fresh tissue for possible molecular diagnosis b. Specific cytology form to be filled out Flow Cytometry 1. Previous communication with ophthalmic pathologist to discuss a. Fresh tissue is critical. b. Adequate sample is essential. c. Geographic proximity to the laboratory Molecular Techniques and Electron Microscopy 1. Previous communication with ophthalmic pathologist to discuss a.
Uses epifluorescence and quantitative, regional differences in the fluorescence ratio of gains/ losses vs control DNA to identify abnormal regions in the genome at a resolution of 20-80 base pairs Differentially labeled test and reference DNAs, hybridized to cloned fragments, genomic DNA or cDNA, which are spotted on a glass slide (the array). The DNA copy number aberrations measured by detecting intensity differences in the hybridization patterns Is y s. 3d Microarray-based CGH (array CGH) th Advantages Disadvantages Quality snap-frozen tissue (optimal) and archival paraffinembedded tissue 1.
2014-2015 Basic and Clinical Science Course (BCSC): Section 4: Ophthalmic Pathology and Intraocular Tumors by American Academy of Ophthalmology, Robert H. Rosa Jr. MD